We use flow cytometry as our main metric in characterizing cells.
For ATP production, we recommend fluorescent or bioluminescent assays.
Our proprietary structure drives cell migration by preventing clumping, stratification, and differentiation amongst cell lines.
The cells can be removed from T-Blocks via trypsinization.
Cell yields of approximately 70% have been achieved through trypsinization of the T-Blocks.
Yes, the T-Blocks are gas permeable.
We have found that 100,000 cells/unit is an ideal cell seeding density to start with a standard size T-Block (1 cm^3).
The T-Blocks are currently for research purposes only. A GMP grade clinical T-Block is under development.
Using a fluorescent dye like Hoechst is the best way to visualize cell nuclei, if you are viewing live cells. The fluorescence helps tremendously when using an epi-fluorescent inverted microscope to view where cells are in the T-Block. If you fix the T-Block and fluorescently immuno-label the cells, you should be able to visualize the cells in the T-Block easily.
Furthermore, if you fix the T-Block, and embed in a cryo-media like OCT, you can section the T-Block to see all aspects of the interior of the T-Block.
Cells can be viewed on the surface of the T-Block with limited light penetration into the T-Block. Rotating the T-Block allows you to see the other regions of the T-Block. Using this method, crude confluence can be assessed and used to determine when to add another T-Block.